LOCUS       sequence-132984-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Rizzo MA, Springer GH, Granada B, Piston DW
  TITLE     An improved cyan fluorescent protein variant useful for FRET.
  JOURNAL   Nat Biotechnol. 2004 Apr . 22(4):445-9.
  PUBMED    14990965
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2004 Apr . 22(4):445-9."
COMMENT     Sequence Label: Cerulean
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..197
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(198..449)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(240..261)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     589..608
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      121 ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcga agcttgagct
      181 cgagatctga gtccggactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      241 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac
      301 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      361 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      421 ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtc gctgatggcg
      481 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      541 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      601 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
      661 aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcac gccccaggtc
      721 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
      781 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
      841 ccgttt
//
