LOCUS       sequence-132985-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses 3*FLAG tagged human NSD3-short with W284A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: MSCV-NSD3-short W284A
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             796..861
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(913..936)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 acnncgagan nnatagaaca gtatactttt atttacattg ataaacagcc tgaagaggct
       61 ttatcccaag naaaaaagag tgttgcctcc aaaaccgaag ttaaaaaaac ccgacgacca
      121 agatctgtgc tgaatactca gccagaacag accaatgcag gggaggtggc ctcctcactc
      181 tcaagtactg aaattcggag acatagccag aggcggcaca caagtgcgga agaggaagag
      241 ccaccgcctg ttaaaatagc ctggaaaact gcggcagcaa ggaaatcctt accagcttcc
      301 attacgatgc acaaagggag cctggatttg cagaagtgta acatgtctcc agttgtgaaa
      361 attgaacaag tgtttgctct tcagaatgct acaggggatg ggaaatttat cgatcaattt
      421 gtttattcaa caaagggaat tggtaacaaa acagaaataa gtgtcagggg gcaagacagg
      481 cttataattt ctacaccaaa ccagagaaat gaaaagccaa cgcagagtgt atcatctcct
      541 gaagcaacat ctggttctac aggctcagta gaaaagaagc aacagagaag atcaattaga
      601 actcgttctg aatcagagaa atccactgag gttgtgccaa agaagaagat caaaaaggag
      661 caggttgaaa cagttcctca ggctacagtg aagactggat tacagaaagg gtcggcggac
      721 cggggagtgc agggctctgt cagattcagt gacagctccg tctccgcagc gattgaggaa
      781 actgtggacg gaggcgatta caaagaccat gacggtgatt ataaagatca tgatatcgat
      841 tacaaggatg acgatgacaa gtgatgagaa ttctaccggg taggggaggc gcttttccca
      901 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      961 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     1021 ggcccnttcg cgcnncnttc tactcctccc ctagtcagga agttcccccn gcccnngagc
     1081 ncnngtcgtg caggacgtnn naatggannn nnnacgnntc ctagtctcgt nnnnngnnnn
     1141 nccnnnnacn nnggaaccgg tagg
//
