LOCUS       sequence-132989-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-D2512H
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc
      121 agtgtgctgg aattctgcag atatccatca cactggcggc cgccatggat tataaagatg
      181 atgatgatat gcttggaacc ggacctgccg ccgccaccac cgctgccacc acatctagca
      241 atgtgagcgt cctgcagcag tttgccagtg gcctaaagag ccggaatgag gaaaccaggg
      301 ccaaagccgc caaggagctc cagcactatg tcaccatgga actccgagag atgagtcaag
      361 aggagtctac tcgcttctat gaccaactga accatcacat ttttgaattg gtttccagct
      421 cagatgccaa tgagaggaaa ggtggcatct tggccatagc tagcctcata ggagtggaag
      481 gtgggaatgc cacccgaatt ggcagatttg ccaactatct tcggaacctc ctcccctcca
      541 atgacccagt tgtcatggaa atggcatcca aggccattgg ccgtcttgcc atggcagggg
      601 acacttttac cgctgagtac gtggaatttg aggtgaagcg agccctggaa tggctgggtg
      661 ctgaccgcaa tgagggccgg agacatgcag ctgtcctggt tctccgtgag ctggccatca
      721 gcgtccctac cttcttcttc cagcaagtgc aacccttctt tgacaacatt tttgtggccg
      781 tgtgggaccc caaacaggcc atccgtgagg gagctgtagc cgcccttcgt gcctgtctga
      841 ttctcacaac ccagcgtgag ccgaaggaga tgcagaagcc tcagtggtac aggcacacat
      901 ttgaagaagc agagaaggga tttgatgaga ccttggccaa agagaagggc atgaatcggg
      961 atgatcggat ccatggagcc ttgttgatcc ttaacgagct ggtccgaatc agcagcatgg
     1021 agggagagcg tctgagagaa gaaatggaag aaa
//
