LOCUS       sequence-133006-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Straathof KC, Pule MA, Yotnda P, Dotti G, Vanin EF, Brenner MK, 
            Heslop HE, Spencer DM, Rooney CM
  TITLE     An inducible caspase 9 safety switch for T-cell therapy.
  JOURNAL   Blood. 2005 Jun 1. 105(11):4247-54.
  PUBMED    15728125
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2005
            Jun 1. 105(11):4247-54."
COMMENT     Sequence Label: pMSCV-F-del Casp9.IRES.GFP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..367
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(719..730)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta cgccaccatg ctcgagggag tgcaggtgga
       61 gactatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca
      121 ctacaccggg atgcttgaag atggaaagaa agttgattcc tcccgggaca gaaacaagcc
      181 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca
      241 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac
      301 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa
      361 actggaatct ggcggtggat ccggagtcga cggatttggt gatgtcggtg ctcttgagag
      421 tttgagggga aatgcagatt tggcttacat cctgagcatg gagccctgtg gccactgcct
      481 cattatcaac aatgtgaact tctgccgtga gtccgggctc cgcacccgca ctggctccaa
      541 catcgactgt gagaagttgc ggcgtcgctt ctcctcgctg catttcatgg tggaggtgaa
      601 gggcgacctg actgccaaga aaatggtgct ggctttgctg gagctggcgc ggcaggacca
      661 cggtgctctg gactgctgcg tggtggtcat tctctctcac ggctgtcagg ccagccacct
      721 gcagttccca ggggctgtct acggcacaga tggatgccct gtgtcggtcg agaagattgt
      781 gaacatcttc aatgggacca gctgccccag cctgggaggg aagcccaagc tctttttcat
      841 cc
//
