LOCUS       sequence-133016-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang 
            XF, Massague J
  TITLE     TGF beta signals through a heteromeric protein kinase receptor 
            complex.
  JOURNAL   Cell. 1992 Dec 11. 71(6):1003-14.
  PUBMED    1333888
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1992 
            Dec 11. 71(6):1003-14."
COMMENT     Sequence Label: pARE-lux
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    309..430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(346..365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     400..419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(825..843)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
       61 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
      121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      181 attacgccag cccaagctac catgataagt aagtaatatt aaggtacgtg gaggttttac
      241 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      301 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      421 atgtatctta tggtactgta actgagctaa cataacccgg gaggtaccga gctcttacgc
      481 gtgctagctc gagcagctga agcttgcatg cctgcaggtc gactctagta tctgctgccc
      541 taaaatgtgt attccatgga aatgtctgcc cttctctccc tagtattgct gccctaaaat
      601 gtgtattcca tggaaatgtc tgcccttctc tccctagtat ctgctgccct aaaatgtgta
      661 ttccatggaa atgtctgccc ttctctccct agagggtata taatggatcc ccgggtaccg
      721 agctcgaatt agcttggcat tccggtactg ttggtaaaat ggaagacgcc aaaaacataa
      781 agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata
      841 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
      901 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga
      961 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtg
//
