LOCUS       sequence-133026-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLJM1-EGFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(21..504)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(57..75)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    553..670
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(783..804)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggaaagtgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg
       61 cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc
      121 tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg
      181 gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg
      241 gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa
      301 ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg
      361 aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg
      421 ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct
      481 tggaaaaggc gcaaccccaa ccccctcgag ccgcggccaa agtggatctc tgctgtccct
      541 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
      601 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
      661 tcttttaaaa ttgtggatga atactgccat ttgtctcgag gtcgagaatt cgaagcttga
      721 gctcgagatc tgagtccgga cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
      781 gtcacgaact ccagcaggac catgtgatcg cgct
//
