LOCUS       sequence-133027-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-STRDHFR
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(526..549)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             566..588
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(631..650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(632..650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cagcgcgcac gcgtggtacc tgtacattag ccggcacgac gctcgaggat
       61 ttcgaaacaa tagctatgcg agttctgcgc atcagcatcg tggaattcgc tgaataccga
      121 ttcccagtca tccggctcgt aatccggaaa atgggtgtcg ccttccactt ctgcatcgat
      181 atgcgtcaga tacagttttt gcgcttttgg caagaactgt tcataaacgc gtccgccgcc
      241 aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcgtcga ccgatttaac
      301 ccaggtaacc cgatcatcgg tcccgggttg actgctgagg ataatatttt tgcggccggg
      361 caaaggccta ccgattgatt cccaggtatg gcgccccata atcacgggtt tatttaaggt
      421 gttgcgttta aaccaggcga gatcggcagg caggttccat ggcatggcgt tttccatgcc
      481 gataacgcga tctaccgcta gcgccgcaat cagactgatg gatcctttct cgaactgcgg
      541 gtggctccag cttgccatat gtatatctcc ttcttaaagt taaacaaaat tattgagctc
      601 tccggagccg gagggaaacc gttgtggtct ccctatagtg agtcgtatta atttcgcggg
      661 atcgatgcat ggtgccaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
      721 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
      781 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
      841 gtatcattac cccatga
//
