LOCUS       sequence-133035-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of myc-tagged Opa1 isoform1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-opa1(isoform 1)-myc
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..101
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(144..173)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(180..209)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(216..245)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(264..293)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(300..329)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(336..365)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(384..413)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(420..449)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(456..485)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattctaga
       61 gtcgcggcct cgaatcacca ctttgtacaa gaaagctggg tttattgaaa atacaagttt
      121 tcgcccccac tagtggatcc gttcaagtct tcttctgaga ttaatttttg ttcaccgttc
      181 aagtcttcct cggagattag cttttgttca ccgttcaaat cttcttcaga aatcaacttt
      241 tgttcacctc tagaggatcc gttcaagtct tcttctgaga ttaatttttg ttcaccgttc
      301 aagtcttcct cggagattag cttttgttca ccgttcaaat cttcttcaga aatcaacttt
      361 tgttcacctc tagaggatcc gttcaagtct tcttctgaga ttaatttttg ttcaccgttc
      421 aagtcttcct cggagattag cttttgttca ccgttcaaat cttcttcaga aatcaacttt
      481 tgttcaccac tcgttcctcc cttctcctgg tgaagagctt caatgaaagc atcaagcttt
      541 tcttgaattt ctctaacttt cttgagatct tctgccagct gaactcgttt gccagtgagc
      601 aatttaacct tcttctcacc gtcttctgca aaatcttcta atacctcttt aacgtttttc
      661 tctagtcgcc taacttcagt gtttgtaagc tgctgcctta atgtattggc agtgatagcg
      721 agcatgcgct gtattcgcca aaacaggacc acgtcattgc attccagctc agaatctata
      781 aaatgcctct ggtagtaata aaaacctctg cgacaaaggt tacaatgatt tagagctgtt
      841 tttaagaaat gtcttctata aacttgatgc caagtatcct taatcaagct tggatcgact
      901 tccactcctc gagactccag gttcttccgg actgtggtaa tctcatcact tgccaggtaa
//
