LOCUS       sequence-133036-         398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD287 (mKate2^SEC^3xMyc
FEATURES             Location/Qualifiers
     source          1..398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     362..381
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttgctcaagg catatatgat gtcaatatct ccggttcggt aagcacaacc atgcagaatg
       61 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
      121 tcgcccggtt tattgaaatg aacggctctt ttgccgacga gaacaggggc tggtgaaatg
      181 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
      241 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
      301 ctgtcagata aagtcccccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
      361 cgcatgatga ccaccgatat ggccagtgtg ccggtctc
//
