LOCUS       sequence-133037-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD287 (mKate2^SEC^3xMyc
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             176..481
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     360..379
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gctcaaggca tatatgatgt caatatctcc ggttcggtaa gcacaaccat gcagaatgaa
       61 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
      121 gcccggttta ttgaaatgaa cggctctttt gccgacgaga acaggggctg gtgaaatgca
      181 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
      241 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
      301 gtcagataaa gtcccccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
      361 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
      421 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
      481 aatgtcaggc tcccttatac acagccagtc tgcaggtcga cctagggagc atcgggagcc
      541 tcaggagcat cgatggtctc cgagctcatt aaagaaaaca tgcatatgaa gctctacatg
      601 gagggaaccg t
//
