LOCUS       sequence-133039-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-opa1(isoform 7DeltaS1)-myc
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    82..106
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(149..178)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(185..214)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(221..250)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(269..298)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(305..334)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(341..370)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(389..418)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(425..454)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(461..490)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
       61 ctagagtcgc ggcctcgaat caccactttg tacaagaaag ctgggtttat tgaaaataca
      121 agttttcgcc cccactagtg gatccgttca agtcttcttc tgagattaat ttttgttcac
      181 cgttcaagtc ttcctcggag attagctttt gttcaccgtt caaatcttct tcagaaatca
      241 acttttgttc acctctagag gatccgttca agtcttcttc tgagattaat ttttgttcac
      301 cgttcaagtc ttcctcggag attagctttt gttcaccgtt caaatcttct tcagaaatca
      361 acttttgttc acctctagag gatccgttca agtcttcttc tgagattaat ttttgttcac
      421 cgttcaagtc ttcctcggag attagctttt gttcaccgtt caaatcttct tcagaaatca
      481 acttttgttc accactcgtt cctcccttct cctggtgaag agcttcaatg aaagcatcaa
      541 gcttttcttg aatttctcta actttcttga gatcttctgc cagctgaact cgtttgccag
      601 tgagcaattt aaccttcttc tcaccgtctt ctgcaaaatc ttctaatacc tctttaacgt
      661 ttttctctag tcgcctaact tcagtgtttg taagctgctg ccttaatgta ttggcagtga
      721 tagcgagcat gcgctgtatt cgccaaaaca ggaccacgtc attgcattcc agctcagaat
      781 ctataaaatg cctctggtag taataaaaac ctctgcgaca aaggttacaa tgatttagag
      841 ctgtttttaa gaaatgtctt ctataaactt gatgccaagt atccttaatc aagcttggat
      901 cgacttccac tcctcgagac tccaggttct tccggactgt ggtaatctca tcacttgcca
      961 ggtaagctgg gtgctcatca ttaaccttca gcatcttctc caactcgttc ttggtttcgt
     1021 tgtgaacaca ctgctcttgg gtccgattct tc
//
