LOCUS       sequence-133058-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-FLAGDHFR
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(526..549)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             563..585
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(628..647)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(629..647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cagcgcgcac gcgtggtacc tgtacattag ccggcacgac gctcgaggat
       61 ttcgaaacaa tagctatgcg agttctgcgc atcagcatcg tggaattcgc tgaataccga
      121 ttcccagtca tccggctcgt aatccggaaa atgggtgtcg ccttccactt ctgcatcgat
      181 atgcgtcaga tacagttttt gcgcttttgg caagaactgt tcataaacgc gtccgccgcc
      241 aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcgtcga ccgatttaac
      301 ccaggtaacc cgatcatcgg tcccgggttg actgctgagg ataatatttt tgcggccggg
      361 caaaggccta ccgattgatt cccaggtatg gcgccccata atcacgggtt tatttaaggt
      421 gttgcgttta aaccaggcga gatcggcagg caggttccat ggcatggcgt tttccatgcc
      481 gataacgcga tctaccgcta gcgccgcaat cagactgatg gatcccttgt catcatcgtc
      541 cttgtagtct cccatatgta tatctccttc ttaaagttaa acaaaattat tgagctctcc
      601 ggagccggag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgcgggatc
      661 gatgcatggt gccaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
      721 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
      781 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
      841 tcattacccc atgaacagaa atccc
//
