LOCUS       sequence-133059-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-STRNDHFR
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(525..545)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(567..590)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             607..629
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(684..703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(685..703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccggatcagc gcgcacgcgt ggtaccaagc tttgtacatt agccggcacg acgctcgagg
       61 atttcgaaac aatagctatg cgagttctgc gcatcagcat cgtggaattc gctgaatacc
      121 gattcccagt catccggctc gtaatccgga aaatgggtgt cgccttccac ttctgcatcg
      181 atatgcgtca gatacagttt ttgcgctttt ggcaagaact gttcataaac gcgtccgccg
      241 ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcgtc gaccgattta
      301 acccaggtaa cccgatcatc ggtcccgggt tgactgctga ggataatatt tttgcggccg
      361 ggcaaaggcc taccgattga ttcccaggta tggcgcccca taatcacggg tttatttaag
      421 gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc atggcatggc gttttccatg
      481 ccgataacgc gatctaccgc tagcgccgca atcagactga tggatccctg gaagtacagg
      541 ttttcaccac caccaccacc agatcctttc tcgaactgcg ggtggctcca gcttgccata
      601 tgtatatctc cttcttaaag ttaaacaaaa ttattgaatt ccaattggag ctctccggag
      661 ccggagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgatg
      721 catggtgcca acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt
      781 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg
      841 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat
      901 taccccatga acagaaatc
//
