LOCUS       sequence-133061-       11100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Estall JL, Ruas JL, Choi CS, Laznik D, Badman M, Maratos-Flier E, 
            Shulman GI, Spiegelman BM
  TITLE     PGC-1alpha negatively regulates hepatic FGF21 expression by 
            modulating the heme/Rev-Erb(alpha) axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 29. 106(52):22510-5.
  PUBMED    20018698
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 29. 106(52):22510-5."
COMMENT     Sequence Label: pAdTrack-CMV FLAG Rev-Erb alpha
FEATURES             Location/Qualifiers
     source          1..11100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   79..181
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        3440..3743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3744..3947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3990..4013
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6021..6142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        6228..6531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6532..6735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6774..6783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6780..6782,6783..6785,6786..7496)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6780..6782,6783..6785,6786..7496)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6825..6846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7086..7105)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7433..7454
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7635..7756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     7899..8049
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   8137..8239
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      8277..8657
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8364..8383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8574..8595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8705..9499)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8705..9499)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8816..8835)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9426..9445
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      10331..10919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10820..10839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11073..11090
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga
       61 tccatgcatg ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg
      121 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      181 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      241 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      301 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      361 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      421 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      481 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      541 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      601 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      661 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
      721 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
      781 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
      841 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
      901 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
      961 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1021 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1081 caagctattg aattcgttta aactccctct caagtctgta tacggggaca cggacagcct
     1141 tttcgtcacc gagcgtggac accggctcat ggaaaccaga ggtaagaaac gcatcaaaaa
     1201 gcatggggga aacctggttt ttgaccccga acggccagag ctcacctggc tcgtggaatg
     1261 cgagaccgtc tgcggggcct gcggcgcgga tgcctactcc ccggaatcgg tatttctcgc
     1321 gcccaagctc tacgccctca aaagtctgca ctgcccctcg tgcggcgcct cctccaaggg
     1381 caagctgcgc gccaagggcc acgccgcgga ggggctggac tatgacacca tggtcaaatg
     1441 ctacctggcc gacgcgcagg gcgaagaccg gcagcgcttc agcaccagca ggaccagcct
     1501 caagcgcacc ctggccagcg cgcagcccgg agcgcacccc ttcaccgtga cccagactac
     1561 gctgacgagg accctgcgcc cgtggaaaga catgaccctg gcccgtctgg acgagcaccg
     1621 actactgccg tacagcgaaa gccgccccaa cccgcgaaac gaggagatat gctggatcga
     1681 gatgccgtag agcaggtgac cgagctgtgg gaccgcctgg aactgcttgg tcaaacgctc
     1741 aaaagcatgc ctacggcgga cggtctcaaa ccgttgaaaa actttgcttc cttgcaagaa
     1801 ctgctatcgc tgggcggcga gcgccttctg gcggatttgg tcagggaaaa catgcgagtc
     1861 agggacatgc ttaacgaagt ggcccccctg ctcagggatg acggcagctg cagctctctt
     1921 aactaccagt tgcagccggt aataggtgtg atttacgggc ccaccggctg cggtaagtcg
     1981 cagctgctca ggaacctgct ttcttcccag ctgatctccc ctaccccgga aaccgttttc
     2041 ttcatcgccc cgcaggtaga catgatcccc ccatctgaac tcaaagcgtg ggaaatgcaa
     2101 atctgtgagg gtaactacgc ccctgggccg gatggaacca ttataccgca gtctggcacc
     2161 ctccgcccgc gctttgtaaa aatggcctat gacgatctca tcctggaaca caactatgac
     2221 gttagtgatc ccagaaatat cttcgcccag gccgccgccc gtgggcccat tgccatcatt
     2281 atggacgaat gcatggaaaa tcttggaggt cacaagggcg tctccaagtt cttccacgca
     2341 tttccttcta agctacatga caaatttccc aagtgcaccg gatacactgt gctggtggtt
     2401 ctgcacaaca tgaatccccg gagggatatg gctgggaaca tagccaacct aaaaatacag
     2461 tccaagatgc atctcatatc cccacgtatg cacccatccc agcttaaccg ctttgtaaac
     2521 acttacacca agggcctgcc cctggcaatc agcttgctac tgaaagacat ttttaggcac
     2581 cacgcccagc gctcctgcta cgactggatc atctacaaca ccaccccgca gcatgaagct
     2641 ctgcagtggt gctacctcca ccccagagac gggcttatgc ccatgtatct gaacatccag
     2701 agtcaccttt accacgtcct ggaaaaaata cacaggaccc tcaacgaccg agaccgctgg
     2761 tcccgggcct accgcgcgcg caaaacccct aaataaagac agcaagacac ttgcttgatc
     2821 caaatccaaa cagagtctgg ttttttattt atgttttaaa ccgcattggg aggggaggaa
     2881 gccttcaggg cagaaacctg ctggcgcaga tccaacagct gctgagaaac gacattaagt
     2941 tcccgggtca aagaatccaa ttgtgccaaa agagccgtca acttgtcatc gcgggcggat
     3001 gaacgggaag ctgcactgct tgcaagcggg ctcaggaaag caaagtcagt cacaatcccg
     3061 cgggcggtgg ctgcagcggc tgaagcggcg gcggaggctg cagtctccaa cggcgttcca
     3121 gacacggtct cgtaggtcaa ggtagtagag tttgcgggca ggacggggcg accatcaatg
     3181 ctggagccca tcacattctg acgcaccccg gcccatgggg gcatgcgcgt tgtcaaatat
     3241 gagctcacaa tgcttccatc aaacgagttg gtgctcatgg cggcggcggc tgctgcaaaa
     3301 cagatacaaa actacataag acccccacct tatatattct ttcccaccct taaccacgcc
     3361 cagatcctct agcagtgata aacgtctaat agtaatcaat tacggggtca ttagttcata
     3421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3961 gtcagatccg ctagagatct ggtaccatgg actacaaaga cgatgacgac aagaccaccc
     4021 tggactccaa caacaacaca ggtggcgtca tcacctacat tggctccagt ggctcctccc
     4081 caagccgcac cagccctgaa tccctctata gtgacaactc caatggcagc ttccagtccc
     4141 tgacccaagg ctgtcccacc tacttcccac catcccccac tggctccctc acccaagacc
     4201 cggctcgctc ctttgggagc attccaccca gcctgagtga tgacggctcc ccttcttcct
     4261 catcttcctc gtcgtcatcc tcctcctcct tctataatgg gagcccccct gggagtctac
     4321 aagtggccat ggaggacagc agccgagtgt cccccagcaa gagcaccagc aacatcacca
     4381 agctgaatgg catggtgtta ctgtgtaaag tgtgtgggga cgttgcctcg ggcttccact
     4441 acggtgtgca cgcctgcgag ggctgcaagg gctttttccg tcggagcatc cagcagaaca
     4501 tccagtacaa aaggtgtctg aagaatgaga attgctccat cgtccgcatc aatcgcaacc
     4561 gctgccagca atgtcgcttc aagaagtgtc tctctgtggg catgtctcga gacgctgtgc
     4621 gttttgggcg catccccaaa cgagagaagc agcggatgct tgctgagatg cagagtgcca
     4681 tgaacctggc caacaaccag ttgagcagcc agtgcccgct ggagacttca cccacccagc
     4741 accccacccc aggccccatg ggcccctcgc caccccctgc tccggtcccc tcacccctgg
     4801 tgggcttctc ccagtttcca caacagctga cgcctcccag atccccaagc cctgagccca
     4861 cagtggagga tgtgatatcc caggtggccc gggcccatcg agagatcttc acctacgccc
     4921 atgacaagct gggcagctca cctggcaact tcaatgccaa ccatgcatca ggtagccctc
     4981 cagccaccac cccacatcgc tgggaaaatc agggctgccc acctgccccc aatgacaaca
     5041 acaccttggc tgcccagcgt cataacgagg ccctaaatgg tctgcgccag gctccctcct
     5101 cctaccctcc cacctggcct cctggccctg cacaccacag ctgccaccag tccaacagca
     5161 acgggcaccg tctatgcccc acccacgtgt atgcagcccc agaaggcaag gcacctgcca
     5221 acagtccccg gcagggcaac tcaaagaatg ttctgctggc atgtcctatg aacatgtacc
     5281 cgcatggacg cagtgggcga acggtgcagg agatctggga ggatttctcc atgagcttca
     5341 cgcccgctgt gcgggaggtg gtagagtttg ccaaacacat cccgggcttc cgtgaccttt
     5401 ctcagcatga ccaagtcacc ctgcttaagg ctggcacctt tgaggtgctg atggtgcgct
     5461 ttgcttcgtt gttcaacgtg aaggaccaga cagtgatgtt cctaagccgc accacctaca
     5521 gcctgcagga gcttggtgcc atgggcatgg gagacctgct cagtgccatg ttcgacttca
     5581 gcgagaagct caactccctg gcgcttaccg aggaggagct gggcctcttc accgcggtgg
     5641 tgcttgtctc tgcagaccgc tcgggcatgg agaattccgc ttcggtggag cagctccagg
     5701 agacgctgct gcgggctctt cgggctctgg tgctgaagaa ccggcccttg gagacttccc
     5761 gcttcaccaa gctgctgctc aagctgccgg acctgcggac cctgaacaac atgcattccg
     5821 agaagctgct gtccttccgg gtggacgccc agtgagcggc cgctcgagcc taagcttcta
     5881 gataagatat ccgatccacc ggatctagat aactgatcat aatcagccat accacatttg
     5941 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     6001 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     6061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6121 ccaaactcat caatgtatct taacgcggag gtttatcgac gatctgctag tgattaatag
     6181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     6241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     6301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     6361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     6421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     6481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     6541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     6601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     6661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     6721 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca
     6781 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6841 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6901 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6961 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     7021 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     7081 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     7141 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     7201 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     7261 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     7321 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     7381 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7441 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
     7501 gactcagatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     7561 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     7621 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7681 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7741 tcatcaatgt atcttaacgc gatcaagcta gcttgctaga ctcgactgac tataataata
     7801 aaacgccaac tttgacccgg aacgcggaaa acacctgaga aaaacacctg ggcgagtctc
     7861 cacgtaaacg gtcaaagtcc ccgcggccct agacaaatat tacgcgctat gagtaacaca
     7921 aaattattca gatttcactt cctcttattc agttttcccg cgaaaatggc caaatcttac
     7981 tcggttacgc ccaaatttac tacaacatcc gcctaaaacc gcgcgaaaat tgtcacttcc
     8041 tgtgtacacc ggcgcacacc aaaaacgtca cttttgccac atccgtcgct tacatgtgtt
     8101 ccgccacact tgcaacatca cacttccgcc acactactac gtcacccgcc ccgttcccac
     8161 gccccgcgcc acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata
     8221 aggtatatta ttgatgatgt taattaagaa ttaattcgat cctgaatggc gaatggacgc
     8281 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8341 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8401 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8461 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8521 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8581 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8641 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aattttaaca
     8701 aaattcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     8761 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     8821 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     8881 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     8941 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
     9001 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     9061 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     9121 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     9181 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     9241 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     9301 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     9361 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     9421 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     9481 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gagcttgatc
     9541 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     9601 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
     9661 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
     9721 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
     9781 gcggactggc tttctacgtg aaaaggatct aggtgaagat cctttttgat aatctcatgg
     9841 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    10501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
