LOCUS       sequence-133075-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of EBFP2 blue fluorescent protein with NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE
  TITLE     Exploration of New Chromophore Structures Leads to the 
            Identification of Improved Blue Fluorescent Proteins.
  JOURNAL   Biochemistry. 2007 May 22;46(20):5904-10. doi: 10.1021/bi700199g
  PUBMED    17444659
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "2007-05-22- 22"; volume: "46"; issue: "20"; 
            pages: "5904-10"
COMMENT     Sequence Label: pEBFP2-Nuc
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    100..221
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(111..130)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     165..184
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(351..371)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(375..395)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(399..419)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
       61 cgaattttaa caaaatatta acgcttacaa tttacgcctt aagatacatt gatgagtttg
      121 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      181 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      241 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
      301 acaaatgtgg tatggctgat tatgatcagt tatctagatc cggtggatcc tacctttctc
      361 ttcttttttg gatctacctt tctcttcttt tttggatcta cctttctctt cttttttgga
      421 tcagctcgag atctgagtcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg
      481 gcggtgcgga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
      541 acggactggg tgctcaggta gtggctgtcg ggcagcagca cggggccgtc gccgatgggg
      601 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctccacgtt gtggcggatc
      661 ttgaagttca ccttgatgcc gttcttctgc ttgacggcca tgatatagat gttgtggctg
      721 ttgaagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgacgccc
      781 ttcagctcga tgcggttcac tagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      841 ta
//
