LOCUS       sequence-133083-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized SpCas9 fused to first 110 amino acids of 
            human GEMININ and chimeric guide RNA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Gutschner T, Haemmerle M, Genovese G, Draetta GF, Chin L
  TITLE     Post-translational Regulation of Cas9 during G1 Enhances 
            Homology-Directed Repair.
  JOURNAL   Cell Rep. 2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 
            10.1016/j.celrep.2016.01.019.
  PUBMED    26854237
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 
            10.1016/j.celrep.2016.01.019."
COMMENT     Sequence Label: pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             93..113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttggaccgg tgccaccatg gactataagg accacgacgg agactacaag gatcatgata
       61 ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc
      121 acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg
      181 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
      241 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
      301 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
      361 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
      421 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
      481 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
      541 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
      601 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
      661 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
      721 tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac
      781 tgagcaagag cagacggctg gaaa
//
