LOCUS       sequence-133091-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-E1799K
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
      121 gtgtgctgga attctgcaga tatccatcac actggcggcc gccatggatt ataaagatga
      181 tgatgatatg cttggaaccg gacctgccgc cgccaccacc gctgccacca catctagcaa
      241 tgtgagcgtc ctgcagcagt ttgccagtgg cctaaagagc cggaatgagg aaaccagggc
      301 caaagccgcc aaggagctcc agcactatgt caccatggaa ctccgagaga tgagtcaaga
      361 ggagtctact cgcttctatg accaactgaa ccatcacatt tttgaattgg tttccagctc
      421 agatgccaat gagaggaaag gtggcatctt ggccatagct agcctcatag gagtggaagg
      481 tgggaatgcc acccgaattg gcagatttgc caactatctt cggaacctcc tcccctccaa
      541 tgacccagtt gtcatggaaa tggcatccaa ggccattggc cgtcttgcca tggcagggga
      601 cacttttacc gctgagtacg tggaatttga ggtgaagcga gccctggaat ggctgggtgc
      661 tgaccgcaat gagggccgga gacatgcagc tgtcctggtt ctccgtgagc tggccatcag
      721 cgtccctacc ttcttcttcc agcaagtgca acccttcttt gacaacattt ttgtggccgt
      781 gtgggacccc aaacaggcca tccgtgaggg agctgtagcc gcccttcgtg cctgtctgat
      841 tctcacaacc cagcgtgagc cgaaggagat gcagaagcct cagtggtaca ggcacacatt
      901 tgaagaagca gagaagggat ttgatgagac cttggccaaa gagaagggca tgaatcggga
      961 tgatcggatc catggagcct tgttgatcct t
//
