LOCUS       sequence-133103-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-TEV-U+36GFP-Cre for E.Coli expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: His-TEV-U_(+36GFP)-Cre
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatca tatgcaccat caccatcacc atgaaaacct gtacttccag
       61 ggcctgtttg atattattaa aaaagtggcg agcgtgattg gcggcctggc tagcggtggt
      121 agcggtggtt ctggtggttc tggtggtagc ggcggtagcg gtggtagcgg tggtagcggt
      181 ggcagcggcg gttctagcaa aggtgaacgt ctgtttcgtg gtaaagtacc gatcttagtg
      241 gaattaaagg gcgacgtgaa cggtcataaa tttagcgtgc gcggcaaagg caaaggtgac
      301 gctacccgtg gtaaattgac cctgaagttt atttgcacaa caggcaaatt acccgttccg
      361 tggcccacct tagtgaccac cctgacctat ggcgttcagt gcttcagtcg ttaccctaaa
      421 cacatgaaac gtcacgattt tttcaaatca gccatgccta aaggatatgt tcaagagcgt
      481 acaatcagct tcaagaagga tggcaaatat aaaacgcgtg cggaagtgaa atttgaaggc
      541 cgcacattag taaatcgtat caaactgaaa ggtcgtgact tcaaagaaaa aggcaacatt
      601 ttaggccata aactgcgtta taactttaat tctcataagg tgtatattac ggccgataaa
      661 cgcaagaatg gtatcaaggc aaaattcaaa attcgccata acgtgaaaga cggcagcgtt
      721 caattagcgg atcattatca acaaaacacg ccgattggtc gcgggcctgt actgttacct
      781 cgcaaccact acctgagcac ccgttctaaa ctgagcaa
//
