LOCUS       sequence-133105-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 4 to 6 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1deltaH4-H6
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(459..479)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(480..497)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             516..538
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    553..577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(577..596)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(578..596)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(680..699)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        905..982
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(1002..1021)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcttac tgggtgttca gttttttggt atactcttcc
      121 agcgcgctca gaaagctcac tttaaagctt tccagcaccg gcagcaggcc ctgacgcaga
      181 tcttccagcg ccggtttcgc tttttcgctc agggtgctca gatgttcggt cgctttcgca
      241 tgatattccg ccagacgcgc accgccgttt tctttcagcg cttccagacg ggccgccaga
      301 cgctgacgca gttcatcgct atacggctgc actttcgctt tcacctcttc cagatctttg
      361 ctcatttcct gacgcaggcc ttcggtttct ttttccaggt tatcccaaaa ttcctgggtc
      421 accgggccca gctgttcacg cagtttgcta aaggtgctgc cctgaaaata caggttttca
      481 tgatgatgat gatgatggct gctgcccatg gtatatctcc ttcttaaagt taaacaaaat
      541 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      601 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      661 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      721 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
      781 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
      841 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat
      901 cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
      961 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     1021 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     1081 aa
//
