LOCUS       sequence-133111-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of His-tagged human Dcp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Wang Z, Jiao X, Carr-Schmid A, Kiledjian M
  TITLE     The hDcp2 protein is a mammalian mRNA decapping enzyme.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8. Epub 2002 Sep 
            6.
  PUBMED    12218187
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-10-1"; volume: "99(20)"; pages: 
            "12663-8. Epub 2002 Sep 6"
COMMENT     Sequence Label: pET28a-hDcp2
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             101..133
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggctagca tgactggtgg
      121 acagcaaatg ggtcgcggat ccatggagac caaacgggtg gagattcccg gcagcgtcct
      181 ggacgatctc tgcagccgat ttattttgca tattcccagc gaggaaagag acaatgcaat
      241 ccgagtgtgt tttcagattg aacttgccca ttggttttac ttggatttct acatgcagaa
      301 cacaccagga ttacctcagt gtgggataag agactttgct aaagctgtct tcagtcattg
      361 tccgtttttg ctgcctcaag gtgaagatgt ggaaaaagtt ttggatgaat ggaaggaata
      421 taaaatggga gtaccaacat atggtgcaat tattcttgat gagacacttg aaaatgtact
      481 actggttcag gggtacctag caaaatcagg ctggggattt ccaaaaggaa aagtaaataa
      541 agaagaagct cctcatgatt gtgctgctag agaggtcttt gaagaaactg gttttgatat
      601 caaagactat atttgtaagg atgattacat tgaacttcga atcaatgacc agcttgctcg
      661 tttgtacatc attccaggaa ttccaaaaga cacaaaattt aacccaaaaa ctagaagaga
      721 aattcggaac attgagtggt tctctattga gaaattgcct tgtcatagaa atgatatgac
      781 ccccaaatcc aaacttggtt tggcacctaa caaatttttt atggccattc cctttatcag
      841 accattaagg gactggcttt ctcgaagatt tggcgattcc tcagacagtg acaatggatt
      901 ttcctcaact ggtagcacgc cggctaaacc cactgtggaa aaattgagtc gaaccaaatt
      961 ccgccacagt cagcagttat ttcctgacgg ttctcctggt gaccagtggg taaagcacag
     1021 gcaaccactg cagcaaaagc catataataa tcattctgaa atgtctgacc tcttaaaagg
     1081 aaagaa
//
