LOCUS       sequence-133113-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     291..310
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     550..571
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      613..622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(629..649)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(653..673)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(677..697)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      700..709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(736..787)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(765..785)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
       61 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgcag ttgaggccga
      121 agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg ccgtggatgg
      181 cgttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      241 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      301 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      361 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc acgccccagc
      421 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      481 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      541 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
      601 cgcccttgct caccatggtg gcgaccggta cctttctctt cttttttgga tctacctttc
      661 tcttcttttt tggatctacc tttctcttct tttttggatc catggtggct agcggatctg
      721 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      781 ttgcgtcaat gggg
//
