LOCUS       sequence-133121-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    57..109
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      613..622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(649..700)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(678..698)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccttgatgat ggccatgtta ttctcctcgc ccttgctcac catggtggcg accggtggat
       61 cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt gagctcgaga gtgctcccgc
      121 cactgccctc cgagggcacc gcggccggac tggcaggcgc cgccggggtg cggggggcag
      181 cgtttcgcgc gcgggcgcgg gccacgccgc tgcaggacac cacgccgtgg cgccggcgga
      241 agcggtccag ccagccgttg gaggcggtga agtcgtccat gcccagctcc tcggctatgc
      301 gcagcgcctt ctccttgagg atgatgccct tgaccggcag gccggcggcg cggatctgct
      361 ggaaccaggc gatgagcaag ccctcgagct tgtcgtaggg agacagcttg ttggtcttgc
      421 ggcaggtgga ggccaccccg tacttgcgct ccgacgccag gatggcgcgc ttgttcttca
      481 ggatcgtgct cagcgtggac ggcgggatgt tgaagcgccg cgcgatctcg cccttgcgta
      541 ggtccgggtt ctcctccacc tcctggatga tccgcgactt ctcccggaac gtcagctgcc
      601 gccgcttggg gcccatggtg gctagcggat ctgacggttc actaaaccag ctctgcttat
      661 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      721 acattttgga aagtcccgtt gatttt
//
