LOCUS       sequence-133123-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Marking retinal neurons with infrared fluorescent proteins (iRFP) to
            preserve photoresponsiveness.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Fyk-Kolodziej B, Hellmer CB, Ichinose T
  TITLE     Marking cells with infrared fluorescent proteins to preserve 
            photoresponsiveness in the retina.
  JOURNAL   Biotechniques. 2014 Nov 1;57(5):245-53. doi: 10.2144/000114228. 
            eCollection 2014 Nov.
  PUBMED    25391913
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.2144/000114228";
            journalName: "Biotechniques"; date: "2014-11-1- 1"; volume: "57"; 
            issue: "5"; pages: "245-53"
COMMENT     Sequence Label: pAAV-CMV-iRFP
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          165..543
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     regulatory      635..644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc
      121 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
      181 gagtctatag gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat
      241 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
      301 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
      361 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
      421 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
      481 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
      541 cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga
      601 ttcgaacatc gattgaattc cccggggatc tgccgccacc atggcggaag gatccgtcgc
      661 caggcagcct gacctcttga cctgcgacga tgagccgatc catatccccg gtgccatcca
      721 accgcatgga ctgctgctcg ccctcgccgc cgacatgacg atcgttgccg gcagcgacaa
      781 ccttcccgaa ctcaccggac tggcgatcgg cgccctgatc ggccgctctg cggccgatgt
      841 cttcgactcg gagacgcaca accgtctgac gatcgccttg gccgagcccg gggcggccgt
      901 cggagcaccg atcactgtcg gcttcacgat gcgaaaggac gcaggcttca tcggctcctg
      961 gcatcgccat gatcagctca tcttcctcga gctcgagcct ccccagcggg acgtcgccga
     1021 gccgcaggcg ttcttccgcc gcaccaacag cgccatccgc cgcctgcagg ccgccgaaac
     1081 cttggaaagc gcctgcgccg ccgcggcgca agaggtgcgg aagattaccg gcttcgatcg
     1141 ggtg
//
