LOCUS       sequence-133126-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of His-tagged human Dcp2 Q147/8 mutant 
            (disrupts the decapping function of Dcp2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Wang Z, Jiao X, Carr-Schmid A, Kiledjian M
  TITLE     The hDcp2 protein is a mammalian mRNA decapping enzyme.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8. Epub 2002 Sep 
            6.
  PUBMED    12218187
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-10-1"; volume: "99(20)"; pages: 
            "12663-8. Epub 2002 Sep 6"
COMMENT     Sequence Label: pET28a-hDcp2 E147Q, E148Q
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..99
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             103..135
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccctctagaa taattttgtt tactttaaga aggagatata ccatgggcag cagccatcat
       61 catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggctag catgactggt
      121 ggacagcaaa tgggtcgcgg atccatggag accaaacggg tggagattcc cggcagcgtc
      181 ctggacgatc tctgcagccg atttattttg catattccca gcgaggaaag agacaatgca
      241 atccgagtgt gttttcagat tgaacttgcc cattggtttt acttggattt ctacatgcag
      301 aacacaccag gattacctca gtgtgggata agagactttg ctaaagctgt cttcagtcat
      361 tgtccgtttt tgctgcctca aggtgaagat gtggaaaaag ttttggatga atggaaggaa
      421 tataaaatgg gagtaccaac atatggtgca attattcttg atgagacact tgaaaatgta
      481 ctactggttc aggggtacct agcaaaatca ggctggggat ttccaaaagg aaaagtaaat
      541 aaagaagaag ctcctcatga ttgtgctgct agagaggtct ttcaacaaac tggttttgat
      601 atcaaagact atatttgtaa ggatgattac attgaacttc gaatcaatga ccagcttgct
      661 cgtttgtaca tcattccagg aattccaaaa gacacaaaat ttaacccaaa aactagaaga
      721 gaaattcgga acattgagtg gttctctatt gagaaattgc cttgtcatag aaatgatatg
      781 acccccaaat ccaaacttgg tttggcacct aacaaatttt ttatggccat tccctttatc
      841 agaccattaa gggactggct ttctcgaaga tttggcgatt cctcagacag tgacaatgga
      901 ttttcctcaa ctggtagcac gccggctaaa cccactgtgg aaaaattgag tcgaaccaaa
      961 ttccgccaca gtcagcagtt atttcctgac ggttctcctg gtgaccagtg ggtaaagcac
     1021 aggcaaccac tgcagcaaaa gccatataat aatcattctg aaatgtctga cctcttaaaa
     1081 ggaaagaatc aaag
//
