LOCUS       sequence-133131-        5295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Bennett AM, Hausdorff SF, O'Reilly AM, Freeman RM, Neel BG
  TITLE     Multiple requirements for SHPTP2 in epidermal growth factor-mediated
            cell cycle progression.
  JOURNAL   Mol Cell Biol 1996 Mar;16(3):1189-202.
  PUBMED    8622663
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1996-03"; volume: "16(3)"; pages: "1189-202"
COMMENT     Sequence Label: pJ3 SHP2 E76A
FEATURES             Location/Qualifiers
     source          1..5295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     231..253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(291..311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        296..625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      476..611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2585..2650
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2780..2800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3225..3359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3262..3281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3316..3335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3390..3494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3495..3563,3564..4355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3713..3732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4526..5114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5015..5034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5268..5285
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgt ggaatgtgtg
      301 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      361 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      421 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
      481 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
      541 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
      601 ttttggaggc ctaggctttt gcaaaagctt cgatcgtcga ctctagagga tccccgggcg
      661 agctcgaatt ccgggcggga ggaacatgac atcgcggaga tggtttcacc caaatatcac
      721 tggtgtggag gcagaaaacc tactgttgac aagaggagtt gatggcagtt ttttggcaag
      781 gcctagtaaa agtaaccctg gagacttcac actttccgtt agaagaaatg gagctgtcac
      841 ccacatcaag attcagaaca ctggtgatta ctatgacctg tatggagggg agaaatttgc
      901 cactttggct gcgttggtcc agtattacat ggaacatcac gggcaattaa aagagaagaa
      961 tggagatgtc attgagctta aatatcctct gaactgtgca gatcctacct ctgaaaggtg
     1021 gtttcatgga catctctctg ggaaagaagc agagaaatta ttaactgaaa aaggaaaaca
     1081 tggtagtttt cttgtacgag agagccagag ccaccctgga gattttgttc tttctgtgcg
     1141 cactggtgat gacaaagggg agagcaatga cggcaagtct aaagtgaccc atgttatgat
     1201 tcgctgtcag gaactgaaat acgacgttgg tggaggagaa cggtttgatt ctttgacaga
     1261 tcttgtggaa cattataaga agaatcctat ggtggaaaca ttgggtacag tactacaact
     1321 caagcagccc cttaacacga ctcgtataaa tgctgctgaa atagaaagca gagttcgaga
     1381 actaagcaaa ttagctgaga ccacagataa agtcaaacaa ggcttttggg aagaatttga
     1441 gacactacaa caacaggagt gcaaacttct ctacagccga aaagagggtc aaaggcaaga
     1501 aaacaaaaac aaaaatagat ataaaaacat cctgcccttt gatcatacca gggttgtcct
     1561 acacgatggt gatcccaatg agcctgtttc agattacatc aatgcaaata tcatcatgcc
     1621 tgaatttgaa accaagtgca acaattcaaa gcccaaaaag agttacattg ccacacaagg
     1681 ctgcctgcaa aacacggtga atgacttttg gcggatggtg ttccaagaaa actcccgagt
     1741 gattgtcatg acaacgaaag aagtggagag aggaaagagt aaatgtgtca aatactggcc
     1801 tgatgagtat gctctaaaag aatatggcgt catgcgtgtt aggaacgtca aagaaagcgc
     1861 cgctcatgac tatacgctaa gagaacttaa actttcaaag gttggacaag ggaatacgga
     1921 gagaacggtc tggcaatacc actttcggac ctggccggac cacggcgtgc ccagcgaccc
     1981 tgggggcgtg ctggacttcc tggaggaggt gcaccataag caggagagca tcatggatgc
     2041 agggccggtc gtggtgcact gcagtgctgg aattggccgg acagggacgt tcattgtgat
     2101 tgatattctt attgacatca tcagagagaa aggtgttgac tgcgatattg acgttcccaa
     2161 aaccatccag atggtgcggt ctcagaggtc agggatggtc cagacagaag cacagtaccg
     2221 atttatctat atggcggtcc agcattatat tgaaacacta cagcgcagga ttgaagaaga
     2281 gcagaaaagc aagaggaaag ggcacgaata tacaaatatt aagtattctc tagcggacca
     2341 gacgagtgga gatcagagcc ctctcccgcc ttgtactcca acgccaccct gtgcagaaat
     2401 gagagaagac agtgctagag tctatgaaaa cgtgggcctg atgcaacagc agaaaagttt
     2461 cagatgagaa aacctgccaa aacttcagca cagaaataga tggtaccaga tctttgtgaa
     2521 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     2581 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     2641 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     2701 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     2761 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     2821 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     2881 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     2941 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     3001 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     3061 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     3121 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3181 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     3361 ctgcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5281 gcgaggaagc ggaag
//
