LOCUS       sequence-133133-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: His-TEV-Aurein1.2_(+36GFP)-LPETG-His
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..57
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agaaggagat atcatatgca ccatcaccat caccatgaaa acctgtactt ccagggcctg
       61 tttgatatta ttaaaaaaat tgcggaaagc tttgctagcg gtggtagcgg tggttctggt
      121 ggttctggtg gtagcggcgg tagcggtggt agcggtggta gcggtggcag cggcggttct
      181 agcaaaggtg aacgtctgtt tcgtggtaaa gtaccgatct tagtggaatt aaagggcgac
      241 gtgaacggtc ataaatttag cgtgcgcggc aaaggcaaag gtgacgctac ccgtggtaaa
      301 ttgaccctga agtttatttg cacaacaggc aaattacccg ttccgtggcc caccttagtg
      361 accaccctga cctatggcgt tcagtgcttc agtcgttacc ctaaacacat gaaacgtcac
      421 gattttttca aatcagccat gcctaaagga tatgttcaag agcgtacaat cagcttcaag
      481 aaggatggca aatataaaac gcgtgcggaa gtgaaatttg aaggccgcac attagtaaat
      541 cgtatcaaac tgaaaggtcg tgacttcaaa gaaaaaggca acattttagg ccataaactg
      601 cgttataact ttaattctca taaggtgtat attacggccg ataaacgcaa gaatggtatc
      661 aaggcaaaat tcaaaattcg ccataacgtg aaagacggca gcgttcaatt agcggatcat
      721 tatcaacaaa acacgccgat tggtcgcggg cctgtactgt tacctcgcaa ccactacctg
      781 agcacccgtt ctaaactgag caa
//
