LOCUS       sequence-133136-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A 
            and L567A substitutions) tagged with EGFP along with shRNA to knock 
            down endogenous TACC3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP(L566,567A)-shTACC3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actataggtc cggtggacag gggtgggccc ccaggcgcgg gaacacctgg gggtgggcct
       61 ggcacaggaa tgtccagtgc tcccaagaaa tcgaactcca caagcttggc ttcccgcgga
      121 cgtcctgagg gagtctcatt tgcaccgtgc atgctgctgg tctcggtggc cacgggcact
      181 ggtctaccag gactgtccct cgcggcgggg tcgaacttga ggtataagga ctgcttcctc
      241 aaggccgact ccttaaacga ggaagttcca aactgctcca ggtaatccac ctccgcgccc
      301 gtgcctagaa cctcagcggg gtctctgaag ctctcctctt tcagctccaa ggcaggctgc
      361 ccctgctgat gggtgggcac tggctcactg cctggacagc ttgtgggaag cgaggtgctg
      421 gcagagttca aggctctctc cttgctctct gctgttgggg tctcggctgc caactgcacc
      481 acaggcgtgt cctccgctga ggctgaatgc agctgctggc tcagacaggg acctggctcc
      541 tccgtggcag gcccagcatg caactgttca gccgctggct ggcccagcct ggtctcaggg
      601 ctttctgggg gctgggcctc actgcaacca gacttggtgt cacctccgaa cgggatgaag
      661 tttgggtcat ccattttgtc ccagtcgagg tggtaagagc cccgagaagc tggcatgggg
      721 gggtcctctc ctgctccgct cctaccgtcg tcctcctcca cctcctgcgg ggccttttgc
      781 tgcctcactg ctgctttcct caagggaggc ttgaggccgg acctctctcc cagtctcctt
      841 ggtgg
//
