LOCUS       sequence-133139-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-HPCDHFR
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(525..560)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     RBS             574..596
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(639..658)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(640..658)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagccggatc agcgcgcacg cgtggtacct gtacattagc cggcacgacg ctcgaggatt
       61 tcgaaacaat agctatgcga gttctgcgca tcagcatcgt ggaattcgct gaataccgat
      121 tcccagtcat ccggctcgta atccggaaaa tgggtgtcgc cttccacttc tgcatcgata
      181 tgcgtcagat acagtttttg cgcttttggc aagaactgtt cataaacgcg tccgccgcca
      241 atcaccatga tttctggtac gtcaccacac gccgcgatgg cttcgtcgac cgatttaacc
      301 caggtaaccc gatcatcggt cccgggttga ctgctgagga taatattttt gcggccgggc
      361 aaaggcctac cgattgattc ccaggtatgg cgccccataa tcacgggttt atttaaggtg
      421 ttgcgtttaa accaggcgag atcggcaggc aggttccatg gcatggcgtt ttccatgccg
      481 ataacgcgat ctaccgctag cgccgcaatc agactgatgg atcctttacc gtcgattaaa
      541 cgtggatcta cctgatcttc agacatatgt atatctcctt cttaaagtta aacaaaatta
      601 ttgagctctc cggagccgga gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat
      661 ttcgcgggat cgatgcatgg tgccaacacc tacatctgta ttaacgaagc gctggcattg
      721 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
      781 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
      841 tttcatcggt atcattaccc catgaacaga aatccc
//
