LOCUS       sequence-133150-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Bell RT, Fu BX, Fire AZ
  TITLE     Cas9 Variants Expand the Target Repertoire in Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2015 Dec 17. pii: genetics.115.185041.
  PUBMED    26680661
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Dec 17. pii: genetics.115.185041."
COMMENT     Sequence Label: pRB1080
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..110
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(148..170)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(442..517)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(519..868)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     874..896
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     888..905
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     889..905
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    913..933
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
       61 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
      121 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
      181 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      241 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgcac tgagagtgca
      301 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
      361 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      421 acgccagaaa tttcacaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      481 tagccttatt ttaacttgct atttctagct ctaaaaccaa gacatctcgc aataggaggt
      541 gtttatatag gggcaacaca agaggcggca gacatctcgt gagtttgaca gagtagtata
      601 aagagtgtgt gtgagagggt gtaggcattt cataacatat tttttgtgga ataattttgg
      661 aaaaaaggtg tttttttaat aatagctttt ataagagttt ctcaaaaatt gcatgctttt
      721 tttgaaaagg ttgacaaatt atagtagtac taaagttgct attttcaggt ctcagcagaa
      781 aattcacaac taaaaataaa atttgcaaaa cagagtgtgc gcctttaagg tgtacagttt
      841 tttattcctt tattgctagt tttttttggt tttcccagtc acgacgttgt aaaacgacgg
      901 ccagtgaatt atcaactatg tataataaag ttgtgagact tttttcttgg cggcacaata
      961 aagtttcctc tttttgttgg gtcactcaac gaacacaata caaatatttg agcgctcgca
     1021 tttttctcaa cctttcgggt ttaaagcact tcaaaatgga attgaagaga gattgtgaaa
     1081 gtacatcaga cagaatcaca ttt
//
