LOCUS       sequence-133161-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U+36GFP-Cre fusion expression in E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: U_(+36GFP)-Cre.ape
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(65..82)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtc
       61 attagtgatg gtggtggtga tgggatccgc cgtcgccatc ttccagcagg cgcaccattg
      121 cccctgtttc actatccagg ttacggatat agttcatgac aatatttaca ttggtccagc
      181 caccagcttg catgatctcc ggtattgaaa ctccagcgcg ggccatatct cgcgcggctc
      241 cgacacgggc actgtgtcca gaccaggcca ggtatctctg accagagtca tccttagcgc
      301 cgtaaatcaa tcgatgagtt gcttcaaaaa tcccttccag ggcgcgagtt gatagctggc
      361 tggtggcaga tggcgcggca acaccatttt ttctgacccg gcaaaacagg tagttattcg
      421 gatcatcagc tacaccagag acggaaatcc atcgctcgac cagtttagtt acccccaggc
      481 taagtgcctt ctctacacct gcagtgctaa ccagcgtttt cgttctgcca atatggatta
      541 acattctccc accgtcagta cgtgagatat ctttaaccct gatcctggca atttcggcta
      601 tacgtaacag ggtgttataa gcaatcccca gaaatgccag attacgtata tcctggcagc
      661 gatcgctatt ttccatgagt gaacgaacct ggtcgaaatc agtgcgttcg aacgctagag
      721 cctgttttgc acgttcaccg gcatcaacgt tttcttttcg gatacgccgc ataaccagtg
      781 aaacagcatt gctgtcactt ggtcgtggca gcc
//
