LOCUS       sequence-133171-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-DHFRCYD
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(604..622)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      618..665
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgggatc catcagtctg attgcggcgc tagcggtaga
       61 tcgcgttatc ggcatggaaa acgccatgcc atggaacctg cctgccgatc tcgcctggtt
      121 taaacgcaac accttaaata aacccgtgat tatggggcgc catacctggg aatcaatcgg
      181 taggcctttg cccggccgca aaaatattat cctcagcagt caacccggga ccgatgatcg
      241 ggttacctgg gttaaatcgg tcgacgaagc catcgcggcg tgtggtgacg taccagaaat
      301 catggtgatt ggcggcggac gcgtttatga acagttcttg ccaaaagcgc aaaaactgta
      361 tctgacgcat atcgatgcag aagtggaagg cgacacccat tttccggatt acgagccgga
      421 tgactgggaa tcggtattca gcgaattcca cgatgctgat gcgcagaact cgcatagcta
      481 ttgtttcgaa atcctcgagc gtcgtgccgg caccgaattt tgcgcttaat gtacaggtac
      541 cacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      601 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      661 ttttgctgaa aggaggaact atatcgggat tagcccgcct aatgagcggg ctttttttta
      721 attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      781 aaccctgata aatgcttcaa taatattgaa a
//
