LOCUS       sequence-133173-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HPCDHFR
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(520..555)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     RBS             569..591
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(628..647)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(629..647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cagcgcgcac gcgttgtaca ttagccggca cgacgctcga ggatttcgaa
       61 acaatagcta tgcgagttct gcgcatcagc atcgtggaat tcgctgaata ccgattccca
      121 gtcatccggc tcgtaatccg gaaaatgggt gtcgccttcc acttctgcat cgatatgcgt
      181 cagatacagt ttttgcgctt ttggcaagaa ctgttcataa acgcgtccgc cgccaatcac
      241 catgatttct ggtacgtcac cacacgccgc gatggcttcg tcgaccgatt taacccaggt
      301 aacccgatca tcggtcccgg gttgactgct gaggataata tttttgcggc cgggcaaagg
      361 cctaccgatt gattcccagg tatggcgccc cataatcacg ggtttattta aggtgttgcg
      421 tttaaaccag gcgagatcgg caggcaggtt ccatggcatg gcgttttcca tgccgataac
      481 gcgatctacc gctagcgccg caatcagact gatggatcct ttaccgtcga ttaaacgtgg
      541 atctacctga tcttcagaca tatgtatatc tccttcttaa agttaaacaa aattatttcc
      601 ggagccggag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgcgggatc
      661 gatgcatggt gccaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
      721 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
      781 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
      841 tcattacccc atgaacagaa atccc
//
