LOCUS       sequence-133175-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HPCNDHFR
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..64
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(661..679)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      675..722
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgtctga agatcaggta gatccacgtt taatcgacgg
       61 taaaggatct ggtggtggtg gtggtgaaaa cctgtacttc cagggatcca tcagtctgat
      121 tgcggcgcta gcggtagatc gcgttatcgg catggaaaac gccatgccat ggaacctgcc
      181 tgccgatctc gcctggttta aacgcaacac cttaaataaa cccgtgatta tggggcgcca
      241 tacctgggaa tcaatcggta ggcctttgcc cggccgcaaa aatattatcc tcagcagtca
      301 acccgggacc gatgatcggg ttacctgggt taaatcggtc gacgaagcca tcgcggcgtg
      361 tggtgacgta ccagaaatca tggtgattgg cggcggacgc gtttatgaac agttcttgcc
      421 aaaagcgcaa aaactgtatc tgacgcatat cgatgcagaa gtggaaggcg acacccattt
      481 tccggattac gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc
      541 gcagaactcg catagctatt gtttcgaaat cctcgagcgt cgtgccggct aatgtacaac
      601 gcgtgcgcgc tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      661 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      721 tgctgaaagg aggaactata tcgggattag cccgcctaat gagcgggctt ttttttaatt
      781 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      841 cctgataaat gcttcaataa tattgaaa
//
