LOCUS       sequence-133176-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-DHFRHIS
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             512..529
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(601..619)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      615..662
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 actttaagaa ggagatatac atatgggatc catcagtctg attgcggcgc tagcggtaga
       61 tcgcgttatc ggcatggaaa acgccatgcc atggaacctg cctgccgatc tcgcctggtt
      121 taaacgcaac accttaaata aacccgtgat tatggggcgc catacctggg aatcaatcgg
      181 taggcctttg cccggccgca aaaatattat cctcagcagt caacccggga ccgatgatcg
      241 ggttacctgg gttaaatcgg tcgacgaagc catcgcggcg tgtggtgacg taccagaaat
      301 catggtgatt ggcggcggac gcgtttatga acagttcttg ccaaaagcgc aaaaactgta
      361 tctgacgcat atcgatgcag aagtggaagg cgacacccat tttccggatt acgagccgga
      421 tgactgggaa tcggtattca gcgaattcca cgatgctgat gcgcagaact cgcatagcta
      481 ttgtttcgaa atcctcgagc gtcgtgccgg ccaccaccac caccaccact aatgtacaac
      541 gcgtgcgcgc tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      601 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      661 tgctgaaagg aggaactata tcgggattag cccgcctaat gagcgggctt ttttttaatt
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaa
//
