LOCUS       sequence-133177-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single donor or transfer plasmid for source of translation cassette 
            for plasmid suite.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pET3aTr
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..91
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(181..199)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      195..242
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(608..613,614..630,631..636))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(663..681)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        749..853
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attggaattc gctagctcta gaaataattt tgtttaactt taagaaggag atatacatat
       61 ggctagcatg actggtggac agcaaatggg tcgcggatcc agatctaagc ttggtaccac
      121 gcgtgcgcgc tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      181 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      241 tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg
      301 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
      361 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
      421 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
      481 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
      541 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
      601 ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
      661 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      721 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      841 aaaaaggaag agtatgagta ttcaacattt
//
