LOCUS       sequence-133181-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sleeping Beauty (SB) transposon with G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Chicaybam L, Barcelos C, Peixoto B, Carneiro M, Limia CG, Redondo P,
            Lira C, Paraguassu-Braga F, Vasconcelos ZF, Barros L, Bonamino MH
  TITLE     An Efficient Electroporation Protocol for the Genetic Modification 
            of Mammalian Cells.
  JOURNAL   Front Bioeng Biotechnol. 2017 Jan 23;4:99. doi: 
            10.3389/fbioe.2016.00099. eCollection 2016.
  PUBMED    28168187
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2017-01-23"; volume: "4:99. doi"; pages:
            " 10.3389/fbioe.2016.00099. eCollection 2016"
COMMENT     Sequence Label: pT3-Neo
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agagcccaca acccctcact cggcgcgcca gtccggatcc accggtgcca ccatggccga
       61 acaggatgga ctgcacgcag gctcccccgc cgcttgggtg gaaagactgt ttggatacga
      121 ctgggctcag cagacaatcg gatgctctga cgcagccgtg ttccggctga gtgctcaggg
      181 gcgccccgtg ctgtttgtca agacagacct gtcaggagca ctgaacgagc tgcaggatga
      241 agcagcccgc ctgagctggc tggctaccac aggagtgcct tgtgctgcag tcctggacgt
      301 ggtcactgag gcaggccgag attggctgct gctgggggaa gtgccaggac aggacctgct
      361 gagctcccac ctggcaccag ctgagaaggt ctccatcatg gcagatgcca tgcggagact
      421 gcatactctg gaccccgcca cctgcccttt cgatcaccag gctaaacatc gcattgagag
      481 ggctcgcacc cgaatggaag caggcctggt ggaccaggac gatctggatg aggaacacca
      541 ggggctggct cctgcagagc tgtttgcacg gctgaaagcc agaatgccag acggagaaga
      601 tctggtggtc acccatggcg acgcttgcct gcccaacatc atggtggaga atggcagatt
      661 cagtgggttt attgattgtg gacgactggg
//
