LOCUS       sequence-133187-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Aurein1.2+36GFP-BirA fusion for E.Coli expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: Aurein1.2_(+36GFP)-BirA
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..80)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagtccatgt gctggcgttc aaatttcgca gcagcggttt ctttaccaga ctcgagtcat
       61 tagtgatggt ggtggtgatg ggatcccctt ttttctgcac tacgcaggga tatttcaccg
      121 cccatccagg gttttattat tccatcctgc tcaagtaata aagccccctg tttgtctatt
      181 ccgcgtgaaa tgccaaatat ttctttatca ccaatgataa gtttcactgg gcgattaata
      241 aaattatcca gcttttccca gcgcgacaga taaggtgcca atccttcttg ttcgaagagt
      301 tccaacgcag cacgtaattc acgtattagc atggccgcca acgtattacg atcgagattg
      361 atccccgctt cctgcagcgt gatccacccc tgattaacga cactctcttc aacacggcgc
      421 attgccatgt tgatcccggc tccaatgact atttgcgccg catcgccagt tttgccagtc
      481 agctcaacca gaatgcctgc cagcttgcga tcctgcagat agaggtcatt aggccattta
      541 acacgaactt tatctgcacc cagcttgcgt aatacttccg ccatcacgat accgataacc
      601 agacttaaac caatcgccgc cgccgggcct tgttccagac gccagaacat cgacaaatat
      661 aagtttgcgc caaaaggcga aaaccatttc cgaccccggc gaccacggcc agcctgctgg
      721 tattctgcaa tgcaagcatc gcccgattta agctctccga tacgatcaag aaggtactga
      781 ttcgtggagt caatcactgg cagcacggct acactaccgc catccagctg acc
//
