LOCUS       sequence-133189-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from 
            two separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Chicaybam L, Barcelos C, Peixoto B, Carneiro M, Limia CG, Redondo P,
            Lira C, Paraguassu-Braga F, Vasconcelos ZF, Barros L, Bonamino MH
  TITLE     An Efficient Electroporation Protocol for the Genetic Modification 
            of Mammalian Cells.
  JOURNAL   Front Bioeng Biotechnol. 2017 Jan 23;4:99. doi: 
            10.3389/fbioe.2016.00099. eCollection 2016.
  PUBMED    28168187
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2017-01-23"; volume: "4:99. doi"; pages:
            " 10.3389/fbioe.2016.00099. eCollection 2016"
COMMENT     Sequence Label: pT3-Neo-EF1a-GFP
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(346..366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtttacatac acttaagttg gagtcattaa aactcgtttt tcaactactc cacaaatttc
       61 ttgttaacaa acaatagttt tggcaagtca gttaggacat ctactttgtg catgacacaa
      121 gtcatttttc caacaattgt ttacagacag attatttcac ttataattca ctgtatcaca
      181 attccagtgg gtcagaagtt tacatacact aagttgactg tgcctttaaa cagcttggaa
      241 aattccagaa aatgatgtca tggctttaga agcttctgat agactaattg acatcatttg
      301 agtcaattgg aggtgtacct gtggatgtat ttcaaggaat tctgtggaat gtgtgtcagt
      361 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatatcg
      421 ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
      481 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
      541 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
      601 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
      661 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttc
//
