LOCUS       sequence-133190-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U+36GFP-BirA for expression in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: U_(+36GFP)-BirA
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(65..82)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtc
       61 attagtgatg gtggtggtga tgggatcccc ttttttctgc actacgcagg gatatttcac
      121 cgcccatcca gggttttatt attccatcct gctcaagtaa taaagccccc tgtttgtcta
      181 ttccgcgtga aatgccaaat atttctttat caccaatgat aagtttcact gggcgattaa
      241 taaaattatc cagcttttcc cagcgcgaca gataaggtgc caatccttct tgttcgaaga
      301 gttccaacgc agcacgtaat tcacgtatta gcatggccgc caacgtatta cgatcgagat
      361 tgatccccgc ttcctgcagc gtgatccacc cctgattaac gacactctct tcaacacggc
      421 gcattgccat gttgatcccg gctccaatga ctatttgcgc cgcatcgcca gttttgccag
      481 tcagctcaac cagaatgcct gccagcttgc gatcctgcag atagaggtca ttaggccatt
      541 taacacgaac tttatctgca cccagcttgc gtaatacttc cgccatcacg ataccgataa
      601 ccagacttaa accaatcgcc gccgccgggc cttgttccag acgccagaac atcgacaaat
      661 ataagtttgc gccaaaaggc gaaaaccatt tccgaccccg gcgaccacgg ccagcctgct
      721 ggtattctgc aatgcaagca tcgcccgatt taagctctcc gatacgatca agaaggtact
      781 gattcgtgga gtcaatcact ggcagcacgg ctacactacc gccatccagc tgacc
//
