LOCUS       sequence-133197-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GFP-labeled mitochondrial outer membrane protein 25.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal
            S, Zoncu R, Chen W, Weinberg RA, Sabatini DM
  TITLE     Stem cells. Asymmetric apportioning of aged mitochondria between 
            daughter cells is required for stemness.
  JOURNAL   Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384. 
            Epub 2015 Apr 2.
  PUBMED    25837514
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1260384"; journalName: "Science"; date: 
            "2015-04-17- 17"; volume: "348"; issue: "6232"; pages: "340-3"
COMMENT     Sequence Label: paGFP-Omp25
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(403..654)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(445..466)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     794..813
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
       61 cgcaacccca accccctcga gccgcggcca aagtggatct ctgctgtccc tgtaataaac
      121 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
      181 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
      241 attgtggatg aatactgcca tttgtctcga ggtcgagaat tctcagagct gctttcggta
      301 tctcacgaag gcccaaactg ctaccagggt aagggcaaac actggcagca gcaccacagc
      361 tacaggaact ccactcggct ctccgtcgcc tcgcccacgc gtcttgtaca gctcgtccat
      421 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      481 gttggggtct ttgctcagtt tggactggtg gctcaggtag tggttgtcgg gcagcagcac
      541 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      601 ctcgatgttg tggcggatct taaagttcgc cttgatgccg ttcttctgct tgtcggccat
      661 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      721 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      781 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      841 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      901 gctgaagcac tgcacgccgt aggaaaaggt ggtcacgagg gtgggccagg gcacgggcag
      961 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     1021 ctcgccgga
//
