LOCUS       sequence-133200-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  +36GFP-LPETG for expression in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: +36GFP-LPETG
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(65..82)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtc
       61 attagtgatg gtggtggtga tgaccggttt ccgggaggct accaccggtc ttgtagcgtt
      121 cgtcgcgtcc gtgcttaatg cctgcagcgg tcacgaattc taacagaacc atgtgatcgc
      181 gtttttcttt cggatctttg ctcagtttag aacgggtgct caggtagtgg ttgcgaggta
      241 acagtacagg cccgcgacca atcggcgtgt tttgttgata atgatccgct aattgaacgc
      301 tgccgtcttt cacgttatgg cgaattttga attttgcctt gataccattc ttgcgtttat
      361 cggccgtaat atacacctta tgagaattaa agttataacg cagtttatgg cctaaaatgt
      421 tgcctttttc tttgaagtca cgacctttca gtttgatacg atttactaat gtgcggcctt
      481 caaatttcac ttccgcacgc gttttatatt tgccatcctt cttgaagctg attgtacgct
      541 cttgaacata tcctttaggc atggctgatt tgaaaaaatc gtgacgtttc atgtgtttag
      601 ggtaacgact gaagcactga acgccatagg tcagggtggt cactaaggtg ggccacggaa
      661 cgggtaattt gcctgttgtg caaataaact tcagggtcaa tttaccacgg gtagcgtcac
      721 ctttgccttt gccgcgcacg ctaaatttat gaccgttcac gtcgcccttt aattccacta
      781 agatcggtac tttaccacg
//
