LOCUS       sequence-133201-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Snap-tag substrates with two different fluorophores (red and green) 
            sequentially to separately label young and old mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal
            S, Zoncu R, Chen W, Weinberg RA, Sabatini DM
  TITLE     Stem cells. Asymmetric apportioning of aged mitochondria between 
            daughter cells is required for stemness.
  JOURNAL   Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384. 
            Epub 2015 Apr 2.
  PUBMED    25837514
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1260384"; journalName: "Science"; date: 
            "2015-04-17- 17"; volume: "348"; issue: "6232"; pages: "340-3"
COMMENT     Sequence Label: Snap-Omp25
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..587
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for mammalian expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     misc_feature    776..893
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aagcagagct ggtttagtga accgtcagat ccgctagcgc catggacaaa gattgcgaaa
       61 tgaaacgtac caccctggat agccctctgg gcaagctgga actgtctggg tgcgaacagg
      121 gcctgcacga gatcaagctg ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc
      181 ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc gcctggctca
      241 acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc ctgcaccacc
      301 cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg aaagtggtga
      361 agttcggaga ggtcatcagc taccagcagc tggccgccct ggccggcaat cccgccgcca
      421 ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc ccctgccacc
      481 gggtggtgtc tagctctggc gccgtggggg gctacgaggg cgggctcgcc gtgaaagagt
      541 ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggtcct gcaggtatag
      601 gcgcgccagg atccacgcgt gggcgaggcg acggagagcc gagtggagtt cctgtagctg
      661 tggtgctgct gccagtgttt gcccttaccc tggtagcagt ttgggccttc gtgagatacc
      721 gaaagcagct ctgagaattc tcgacctcga gacaaatggc agtattcatc cacaatttta
      781 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      841 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      901 attacaggga cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt
      961 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     1021 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     1081 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cc
//
