LOCUS       sequence-133205-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses tagged 4EBP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pRK5-HA-GST-4EBP1-Low GC
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(505..527)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gctgcataac aagttgggcc atggcggcca agctggggat ccttacttac ttagcggccg
       61 cttaaatatc catttcaaac tgagattctt caccacctgc acgtttatct tctggagagt
      121 tacgtaaatg agactgagat gcttccattg gtggttcatc agaagatgga gaagtaacac
      181 ctggaatagt tggtaaatca cgtggtggag ttttagtaac tggagagtta cgacattcca
      241 ttaaaaattt acgatcgtag atgatacgag taccacctgg agtagtagaa aataaagtac
      301 cacctggagt agtagaataa tcacctggtg gtaactgaac accatcacct aaaacaacac
      361 gacgagttgc tggaattgca cgagatggag tctgagaaca agaagaacca ccagacatcg
      421 tcgacccaga tccacctgaa cctcctgatc caccagaacc tcccctcgac ccaccacctc
      481 caccttttgg aggatggtcg ccaccaccaa acgtggcttg ccagccctgc aaaggccatg
      541 ctatatactt gctggatttc aagtacttat caatttgtgg gatagcttca atacgttttt
      601 taaaacaaac taattttggg aacgcatcca ggcacattgg gtccatgtat aaaacaacat
      661 caagagcgtc atacaacatg aagtcaggat gggttacatg atcaccattt aaatatgttt
      721 tatgacataa acgatcttcg aacattttca gcatttcagg tagcttgcta agaaaatcaa
      781 ctttgagagt ttcaaagtct ttactatatg caattctcga aacaccgtat ctaatatcca
      841 aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt tggacaacca cccaacatgt
      901 tgtgcttgtc agctatataa cgtatgatgg ccatagactg tgttaattta acatcaccat
      961 caatataata aaggaagatt gggaaactcc aaacccaatt caaacttttt gtttcgccat
     1021 ttatcacctt catc
//
