LOCUS       sequence-133206-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U_+36GFP-Cys fusion expression in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: U_(pos36GFP)-Cys
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..47)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(66..83)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc agactcgagt
       61 cattagtgat ggtggtggtg atgggatcct ccgcatccgc taccaccggt cttgtagcgt
      121 tcgtcgcgtc cgtgcttaat gcctgcagcg gtcacgaatt ctaacagaac catgtgatcg
      181 cgtttttctt tcggatcttt gctaagttta gaacgggtgc tcaggtagtg gttgcgaggt
      241 aacagtacag gcccgcgacc aatcggcgtg ttttgttgat aatgatccgc taattgaacg
      301 ctgccgtctt tcacgttatg gcgaattttg aattttgcct tgataccatt cttgcgttta
      361 tcggccgtaa tatacacctt atgagaatta aagttataac gcagtttatg gcctaaaatg
      421 ttgccttttt ctttgaagtc acgacctttc agtttgatac gatttactaa tgtgcggcct
      481 tcaaatttca cttccgcacg cgttttatat ttgccatcct tcttgaagct gattgtacgc
      541 tcttgaacat atcctttagg catggctgat ttgaaaaaat cgtgacgttt catgtgttta
      601 gggtaacgac tgaagcactg aacgccatag gtcagggtgg tcactaaggt gggccacgga
      661 acgggtaatt tgcctgttgt gcaaataaac ttcagggtca atttaccacg ggtagcgtca
      721 cctttgcctt tgccgcgcac gctaaattta tgaccgttca cgtcgccctt taattccact
      781 aagatcggta ctttaccacg aa
//
