LOCUS       sequence-133209-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter 
            removed).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Cherry PD, White LK, York K, Hesselberth JR
  TITLE     Genetic bypass of essential RNA repair enzymes in budding yeast.
  JOURNAL   RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 
            Dec 6.
  PUBMED    29212664
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.061788.117"; journalName: "RNA"; date: "2018-03"; 
            volume: "24"; issue: "3"; pages: "313-323"
COMMENT     Sequence Label: pAG424-ccdB
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..268)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     234..252
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     270..286
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     271..287
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    320..444
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(485..790)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(587..606)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gggcgaattg ggtaccggcc gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
       61 aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
      121 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
      181 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
      241 tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata agcttgatat
      301 cgaattcctg cagcccatca ccactttgta caagaaagct gaacgagaaa cgtaaaatga
      361 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      421 aacacaacat atccagtcac tatggtcgac ctgcagactg gctgtgtata agggagcctg
      481 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
      541 tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca
      601 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat
      661 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
      721 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
      781 taaactgcat ttcaccagcc cctgttctcg tcagcaaaag agccgttcat ttcaataaac
      841 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
      901 cgggcttcat tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct
      961 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
     1021 tctttttgac atacttcggg
//
