LOCUS       sequence-133210-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter 
            removed).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Cherry PD, White LK, York K, Hesselberth JR
  TITLE     Genetic bypass of essential RNA repair enzymes in budding yeast.
  JOURNAL   RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 
            Dec 6.
  PUBMED    29212664
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.061788.117"; journalName: "RNA"; date: "2018-03"; 
            volume: "24"; issue: "3"; pages: "313-323"
COMMENT     Sequence Label: pAG424-ccdB
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    336..383
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tatgatccaa tatcaaagga aatgatagca ttgaaggatg agactaatcc aattgaggag
       61 tggcagcata tagaacagct aaagggtagt gctgaaggaa gcatacgata ccccgcatgg
      121 aatgggataa tatcacagga ggtactagac tacctttcat cctacataaa tagacgcata
      181 taagtacgca tttaagcata aacacgcact atgccgttct tctcatgtat atatatatac
      241 aggcaacacg cagatatagg tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt
      301 gcattttcgg aagcgctcgt tttcggaaac gctttgaagt tcctattccg aagttcctat
      361 tctctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc
      421 actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc
      481 gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta
      541 tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt
      601 ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat
      661 agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca
      721 aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt
      781 ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc
      841 ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc
      901 tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta
      961 cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat
     1021 ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgt
//
