LOCUS       sequence-133211-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Gateway TRP1 2micron vector, no promoter (GPD1 promoter 
            removed).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Cherry PD, White LK, York K, Hesselberth JR
  TITLE     Genetic bypass of essential RNA repair enzymes in budding yeast.
  JOURNAL   RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 
            Dec 6.
  PUBMED    29212664
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.061788.117"; journalName: "RNA"; date: "2018-03"; 
            volume: "24"; issue: "3"; pages: "313-323"
COMMENT     Sequence Label: pAG424-ccdB
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..157
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(182..187,188..205,206..212)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             266..925
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(332..351)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taaagggaac aaaagctggt ggatccccca tcacaagttt gtacaaaaaa gctgaacgag
       61 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      121 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg
      181 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag
      241 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      301 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      361 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      421 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      481 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      541 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      601 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
      661 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
      721 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
      781 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
      841 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
      901 cgatgagtgg cagggcgggg cgtaaacgcc gtggatccgg cttactaaaa gccagaatac
//
