LOCUS       sequence-133214-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae 
            introns.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Cherry PD, White LK, York K, Hesselberth JR
  TITLE     Genetic bypass of essential RNA repair enzymes in budding yeast.
  JOURNAL   RNA. 2018 Mar;24(3):313-323. doi: 10.1261/rna.061788.117. Epub 2017 
            Dec 6.
  PUBMED    29212664
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.061788.117"; journalName: "RNA"; date: "2018-03"; 
            volume: "24"; issue: "3"; pages: "313-323"
COMMENT     Sequence Label: pAG424-10X-tRNA
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(73..135)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
ORIGIN
        1 ggtatcgata agcttgatat cgaattcctg cagcccatca ccactttgta caagaaagct
       61 gggtatcgat tttctttctg tatcgcaaat aagtgaaaat taaaaaagaa agattaaatt
      121 ggaagttgga tatgggaaga aacaaagtga gagctaaggg attcgaaccc ttgcatccga
      181 agatatcaga gcctaaatct gacgccttaa accactcggc caaactccct actatttgta
      241 agaaacaaag cgacaccagc aggatttgaa ccagcgcggg cagagcccaa gagatttcga
      301 gtctctcgcc ttaaccactc ggccatagtg cctactattt gtaagaaaca aagggggcga
      361 gctgggaatt gaacccaggg cctctcgcac ccaaagcgag aatcatacca ctagaccaca
      421 cgccctacta tttgtaagaa acaaagtgaa acggacagga attgaacctg caacccttcg
      481 atttggagtc gaaagctcta ccattgagcc accgcttcta ctatttgtaa gaaacaaagt
      541 gctcgaggtg gggtttgaac ccacgacggt cgcgttataa gcacgaagct ctaaccactg
      601 agctacacga gctactattt gtaagaaaca aagcgtcaca gacaggattc gaacctgcgc
      661 aggtaaaacc caatgcctta gcagggcatc gccttaacca ctcggccact gggactacta
      721 tttgtaagaa acaaagctcc tcataggggg ctcgaacccc tgacatttcg gttaaaagcc
      781 gaacgctcta ccaactgagc taacaaggat actatttgta agaaacaaag tggttgctaa
      841 gagattcgaa ctcttgcatc ttacgatacc tgagcttgaa tcaggcgcct tagaccgctc
      901 ggccaaacaa cctactattt gtaagaaaca aagtgcgaat tctgtggatc gaacacagga
      961 cctccagatc tttag
//
