LOCUS       sequence-133216-         285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episomal plasmid encoding  5 gRNAS targeting human OCT4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski 
            T
  TITLE     Conditionally Stabilized dCas9 Activator for Controlling Gene 
            Expression in Human Cell Reprogramming and Differentiation.
  JOURNAL   Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 
            10.1016/j.stemcr.2015.08.001.
  PUBMED    26352799
REFERENCE   2  (bases 1 to 285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.08"; journalName: "Stem Cell Reports"; date: 
            "2015-09-8- 8"; volume: "5"; issue: "3"; pages: "448-59"
COMMENT     Sequence Label: GG-EBNA-OCT4
FEATURES             Location/Qualifiers
     source          1..285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(55..88)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atttcgaggt cgagatccta agcttggctg gacgtaaact cctcttcaga cctaataact
       61 tcgtatagca tacattatac gaagttatat taagggttat tgaatatgat cggaattggg
      121 cgtcgggcga cctttggtcg cccggccgca gtgagcgagc gagcgcgcag agagggagtg
      181 gccaactcca tcactagggg ttcctgcggc cgctcgttag gaaacagcta tgaccatgaa
      241 aaaaagcacc gactcggtgc cactttttca agttgataac ggact
//
