LOCUS       sequence-133247-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the restriction enzyme IPpoI under the control of Shield1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Goldstein M, Derheimer FA, Tait-Mulder J, Kastan MB
  TITLE     Nucleolin mediates nucleosome disruption critical for DNA 
            double-strand break repair.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Sep 30.
  PUBMED    24082117
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Sep 30."
COMMENT     Sequence Label: pCL20C-ddIPpoI
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(74..76,77..397)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             413..439
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt ctgcagtcga cggtaccgcg ggcccgggat
       61 ccaccggtgg atcatgggag tgcaggtgga gactatctcc ccaggagacg ggcgcacctt
      121 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa
      181 agttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt
      241 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac
      301 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc
      361 cactctcgtc ttcgatgtgg agcttctaaa accggaagaa ttcaccatgg catacccata
      421 cgatgttcca gattacgcta gcggatctcg aaatgaaatg ggtgcttcag gagacatgag
      481 ggctgccaac ctttggccaa gccctcttgt gattaagcac actaagaaga atagccctgc
      541 cttgtccttg acagctgacc agatggtcag tgccttgttg gatgctgaac cgcccatgat
      601 ctattctgaa tatgatcctt ctagaccctt cagtgaagcc tcaatgatgg gcttattgac
      661 caacctagca gatagggagc tggttcatat gatcaactgg gcaaagagag tgccaggctt
      721 tggggacttg aatctccatg atcaggtcca ccttctcgag tgtgcctggc tggagattct
      781 gatgattggt ctcgtctggc gctccttgga acacccgggg aagctcctgt ttgctcctaa
      841 cttgctcctg gacaggaatc aaggtaaatg tgtggaaggc atggtggaga tctttgacat
      901 gttgcttgct acgtcaagtc ggttccgcat gatgaacctg cagggtgaa
//
